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Transgenics - Dna Transfer, Use Of Transgenics

cell genetic genes organisms

The term transgenics refers to the process of transferring genetic information from one organism to another. By introducing new genetic material into a cell or individual, a transgenic organism is created that has new characteristics it did not have before. The genes transferred from one organism or cell to another are called transgenes. The development of biotechnological techniques has led to the creation of transgenic bacteria, plants, and animals that have great advantages over their natural counterparts and sometimes act as living machines to create therapeutics for the treatment of disease. Despite the advantages of transgenics, some people have great concern regarding the use of transgenic plants as food, and with the possibility of transgenic organisms escaping into the environment where they may upset ecosystem balance.

All of the cells of every living thing on Earth contain DNA (deoxyribonucleic acid). DNA is a complex and long molecule composed of a sequence of smaller molecules, called nucleotides, linked together. Nucleotides are nitrogen-containing molecules, called bases that are combined with sugar and phosphate. There are four different kinds of nucleotides in DNA. Each nucleotide has a unique base component. The sequence of nucleotides, and therefore of bases, within an organism's DNA is unique. In other words, no two organisms have exactly the same sequence of nucleotides in their DNA, even if they belong to the same species or are related. DNA holds within its nucleotide sequence information that directs the activities of the cell. Groups, or sets of nucleotide sequences that instruct a single function are called genes.

Much of the genetic material, or DNA, of organisms is coiled into compact forms called chromosomes. Chromosomes are highly organized compilations of DNA and protein that make the long molecules of DNA more manageable during cell division. In many organisms, including human beings, chromosomes are found within the nucleus of a cell. The nucleus is the central compartment of the cell that houses genetic information and acts as a control center for the cell. In other organisms, such as bacteria, DNA is not found within a nucleus. Instead, the DNA (usually in the form of a circular chromosome) is free within the cell. Additionally, many cells have extrachromosomal DNA that is not found within chromosomes. The mitochondria of cells, and the chloroplasts of plant cells have extrachromosomal DNA that help direct the activities of these organelles independent from the activities of the nucleus where the chromosomes are found. Plasmids are circular pieces of extrachromosomal DNA found in bacteria that are extensively used in transgenics.

DNA, whether in chromosomes or in extrachromosomal molecules, uses the same code to direct cell activities. The genetic code is the sequence of nucleotides in genes that is defined by sets of three nucleotides. The genetic code itself is universal, meaning it is interpreted the same way in all living things. Therefore, all cells use the same code to store information in DNA, but have different amounts and kinds of information. The entire set of DNA found within a cell (and all of the identical cells of a multicellular organism) is called the genome of that cell or organism.

The DNA of chromosomes within the cellular genome is responsible for the production of proteins. Proteins have many varied and important functions, and in fact help determine the major characteristics of cells and whole organisms. As enzymes, proteins carry out thousands of kinds of chemical reactions that make life possible. Proteins also act as cell receptors and signal molecules, which enable cells to communicate with one another, to coordinate growth and other activities important for wound healing and development. Thus, many of the vital activities and characteristics that define a cell are really the result of the proteins that are present. The proteins, in turn, are determined by the genome of the organism.

Because the genetic code is universal (same for all known organisms), and because genes determine characteristics of organisms, the characteristics of one kind of organism can be transferred to another. If genes from an insect, for example, are placed into a plant in such a way that they are functional, the plant will gain characteristics of the insect. The insect's DNA provides information on how to make insect proteins within the plant because the genetic code is interpreted in the same way. That is, the insect genes give new characteristics to the plant. This very process has already been performed with firefly genes and tobacco plants. Firefly genes were transferred into tobacco plants, which created new tobacco plants that could glow in the dark. This amazing artificial genetic mixing, called recombinant biotechnology, is the crux of transgenics. The organisms that are created from mixing genes from different sources are transgenic. The glow-in-the-dark tobacco plants in the previous example, then, are transgenic tobacco plants.

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