# Electrophoresis

## Electrophoretic Theory

Electrophoretic separations are based upon the fact that the electrical **force** (F) on a charged particle (ion) in an electrical field (E) is proportional to the charge of the particle (q), or F = qE (Eq 1).

The **migration** of the charged particle in the electric field, called the electrophoretic mobility (μ), is defined as μ = *v*/E = q/*f* (Eq 2), where *v* is the **velocity** of the charged particle and *f* is a complex term called the frictional **coefficient**. The frictional coefficient relates to the size and the shape of the particle. From equation (2) it can be seen that electrophoretic mobility decreases for larger particles and increases with higher charge.

## Additional topics

Science EncyclopediaScience & Philosophy: *Dysprosium* to *Electrophoresis - Electrophoretic Theory*Electrophoresis - Electrophoretic Theory, Methodology And Applications, Gel Electrophoresis, Gel Electrophoresis Of Dna, Gel Electrophoresis Of Proteins